Reactions of Some Grapevine Rootstock Cuttings to Mutagenic Applications

Grapevine (Vitis spp.) is one of the most important socio-economically plants in global scale, and need for its breeding increasing. In viticulture, mutation more promising than crossbreeding new genotypes from natural genetic diversity. Polyploid outperform their diploid relatives several respects. this study, efficacy oryzalin N2O mutagens induction polyploidy was investigated by applying different doses durations to forced cuttings 41B [Chasselas vinifera L.) × Vitis berlandieri Planch)] Fercal [(Vitis x berlandieri) 333 EM] rootstocks. LD50 values mutagen applications were determined, morphological cytological effects examined macroscopic, microscopic, methods. Application time dose increase decreased values. As a result applications, leaf thickness chlorophyll content surviving increased. Applications increased stomatal sizes, density, chloroplast numbers, thicknesses partially SPAD It determined that they not polyploid confirmation test performed with flow cytometry (FC) analyses 4 1 samples assumed be mutant stoma examinations. After that, it thought would appropriate try vegetative material actively dividing cells, such as nodal cuttings, studies obtaining individuals on grapevine rootstocks